hHpr1-p84-Thoc1 Antibody (S05-1A8)

Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (S05-1A8) [NBP3-14940] - Immunofluorescence of hHpr1-p84-Thoc1 (green) in Hela cells using hHpr1-p84-Thoc1 Rabbit mAb at dilution 1/50, and DAPI (blue)

Western Blot: hHpr1-p84-Thoc1 Antibody (S05-1A8) [NBP3-14940] - Western blot in Jurkat, Hela, COS7 cell lysates using NBP3-14940 (1:1000 diluted). Predicted band size: 76KDa. Observed band size: 84KDa.

Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (S05-1A8) [NBP3-14940] - Immunofluorescence of hHpr1-p84-Thoc1 (green) in Hela cells using NBP3-14940 at dilution 1/50, and DAPI (blue)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: S05-1A8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

hHpr1-p84-Thoc1 Antibody (S05-1A8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human hHpr1-p84-Thoc1 (Uniprot#: Q96FV9)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: THOC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20-1/50
  • Western Blot 1/1000-1/5000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for hHpr1-p84-Thoc1 Antibody (S05-1A8)

• HPR1THO complex subunit 1
• hTREX84
• Nuclear matrix protein p84
• P84
• P84N5
• THO complex 1
• tho1

Background

P84/N5-5E10 recognizes an 84kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THOC1