HHEX Antibody (2018B) [Unconjugated]

Images

 
A549 human lung carcinoma cell line was stained with Rabbit Anti-Human/Mouse/Rat HHEX Monoclonal Antibody (Catalog # MAB83771, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), ...read more
HHEX was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Rabbit Anti-Human/Mouse/Rat HHEX Polyclonal Antibody (Catalog # MAB83771) at 3 µg/mL for 3 hours at room temperature. ...read more
HHEX was detected in immersion fixed iBJ6 iPS cell line differentiated into hepatocytes using Rabbit Anti-Human/Mouse/Rat HHEX Polyclonal Antibody (Catalog # MAB83771) at 2 µg/mL for 3 hours at room temperature. ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, BaF3 mouse pro-B cell line, and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
2018B
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

HHEX Antibody (2018B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human HHEX
Thr111-Gly270
Accession # Q03014
Specificity
Detects human HHEX in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 2-25 ug/mL
Publications
Read Publication using
MAB83771 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HHEX Antibody (2018B) [Unconjugated]

  • hematopoietically expressed homeobox
  • Hex
  • HEXHMPH
  • HHEX
  • HMPH
  • Homeobox protein HEX
  • Homeobox protein PRH
  • HOX11L-PEN
  • PRH
  • PRHhematopoietically-expressed homeobox protein HHEX
  • PRHX
  • PRHXhomeobox, hematopoietically expressed
  • proline-rich homeodomain-containing transcription factor

Background

Hematopoietically-expressed homeobox protein (HHEX), also known as HEX, PRH and PRHX, is a 35-40 kDa member of the Homeobox family of transcription factors.  Family members are distinguished by an evolutionarily conserved DNA-binding homeodomain of 60 amino acids (aa), which for HHEX spans aa 137-196.  Human HHEX was initially isolated from hematopoietic tissue, and is present in several hematopoietic progenitors, where its expression is down-regulated during terminal cell differentiation.  HHEX is also expressed in the anterior visceral endoderm during early mouse development, and in some adult tissues of endodermal origin, including liver, lung and thyroid.  HHEX knockout in mice is embryonic lethal, with impaired forebrain, liver and thyroid development.  Human HHEX is 270 aa in length, and over aa 111-270, shares 93% and 95% identity with mouse and rat HHEX, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HHEX Antibody (MAB83771)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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(1)
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB83771U
Alexa Fluor 405 FAB83771V
Alexa Fluor 488 FAB83771G
Alexa Fluor 594 FAB83771T
Alexa Fluor 647 FAB83771R
Alexa Fluor 700 FAB83771N
Alexa Fluor 750 FAB83771S

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