HGFR/c-MET [p Tyr1230, p Tyr1234, p Tyr1235] Antibody

Product Discontinued

HGFR/c-MET [p Tyr1230, p Tyr1234, p Tyr1235] Antibody Summary

• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. The sequence is conserved in mouse and rat.
• Modification: p Tyr1230, p Tyr1234, p Tyr1235
• Localization: Type I membrane protein
• Specificity: The phosphospecific that has been generated does not distinguish between the dually (pYpY 1234/1235) and triply (pYpYpY1230/1234/1235) phosphorylated forms of c-Met, both of which are likely to represent activated forms of this receptor.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MET
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot
• Application Notes: Detects a band of approximately 169 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human and Mouse. Expected to cross-react with Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HGFR/c-MET [p Tyr1230, p Tyr1234, p Tyr1235] Antibody

• AUTS9
• cMET
• c-MET
• EC 2.7.10
• EC 2.7.10.1
• hepatocyte growth factor receptor
• HGF R
• HGF receptor
• HGF/SF receptor
• HGFR
• Met (c-Met)
• met proto-oncogene (hepatocyte growth factor receptor)
• met proto-oncogene tyrosine kinase
• MET
• oncogene MET
• Proto-oncogene c-Met
• RCCP2
• Scatter factor receptor
• SF receptor
• Tyrosine-protein kinase Met

Background

Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MET
• Entrez:
  • 4233(Human)
  • 17295(Mouse)