HEY2 Antibody

Product Discontinued

HEY2 Antibody Summary

• Immunogen: Peptide with sequence HASESTPCRLSTTS corresponding to internal region according to NP_036391.1.
• Epitope: HASESTPCRLSTTS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HEY2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments in human brain (cerebellum), mouse and rat brain lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HEY2 Antibody

• BHLHB32
• bHLHb32HES-related repressor protein 2
• cardiovascular basic helix-loop-helix factor 1
• Cardiovascular helix-loop-helix factor 1
• CHF1
• Class B basic helix-loop-helix protein 32
• GRLGRIDLOCK
• Hairy and enhancer of split-related protein 2
• hairy/enhancer-of-split related with YRPW motif 2
• hairy/enhancer-of-split related with YRPW motif protein 2
• Hairy-related transcription factor 2
• hCHF1
• HERP
• HERP1hHRT2
• HESR2
• HESR-2
• HES-related repressor protein 1
• HRT2
• HRT-2
• MGC10720
• Protein gridlock homolog

Background

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HEY2 Antibody (NBP1-52173)(1)

Publication using NBP1-52173

• Tang Y, Urs S, Liaw L. Hairy-related transcription factors inhibit Notch-induced smooth muscle alpha-actin expression by interfering with Notch intracellular domain/CBF-1 complex interaction with the CBF-1-binding site. Circ Res 2008-03-01 [PMID: 18239137]

Bioinformatics

• Gene Symbol: HEY2
• Entrez:
  • 23493(Human)
  • 15214(Mouse)
  • 155430(Rat)
• Uniprot:
  • NP_036391.1(Human)