Hexosaminidase A/HEXA Antibody

Western Blot: Hexosaminidase A/HEXA Antibody [NBP2-30997] - Analysis in control (vector only transfected HEK293T lysate) and HEXA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP2-30997] - Staining of human skin shows no positivity in squamous epithelial cells as expected.

Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP2-30997] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP2-30997] - Staining of human lung shows strong granular cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP2-30997] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

Product Discontinued

Hexosaminidase A/HEXA Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LESFYIQTLLDIVSSYGKGYVVWQEVFDNKVKIQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYVVEPLAFE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HEXA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Hexosaminidase A/HEXA Protein (NBP2-30997PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%), Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Hexosaminidase A/HEXA Antibody

• beta-hexosaminidase subunit alpha
• Beta-N-acetylhexosaminidase subunit alpha
• EC 3.2.1
• EC 3.2.1.52
• HEXA
• hexosaminidase A (alpha polypeptide)
• Hexosaminidase A
• Hexosaminidase subunit A
• MGC99608
• N-acetyl-beta-glucosaminidase subunit alpha
• TSD

Background

HEXA encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HEXA
• Uniprot:
  • P06865()