Hexokinase 2 Antibody (1A7)

Images

 
Western Blot: Hexokinase 2 Antibody (1A7) [NBP1-04296] - A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase I, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Hexokinase 2 Antibody (1A7) Summary

Immunogen
Recombinant human Hexokinase 2 (1-917aa) purified from E. coli
Specificity
Anti-human Hexokinase 2 mAb, clone 1A7, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hexokinase 2 protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HK2
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:3000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Hexokinase 2 Antibody (1A7)

  • DKFZp686M1669
  • EC 2.7.1
  • Hexokinase 2
  • Hexokinase type II
  • HK2
  • HKII
  • HXK2
  • Muscle form hexokinase
  • muscle

Background

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes (types 1 to 4 hexokinases) have been identified. These enzymes are found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HK2
Entrez
Uniprot