Hexim 1 Antibody

Western Blot: Hexim 1 Antibody [NB100-55391] - Staining of human (A) and mouse (B) heart lysates (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody [NB100-55391] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: Hexim 1 Antibody [NB100-55391] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Hexim 1 Antibody Summary

• Description: Novus Biologicals Goat Hexim 1 Antibody (NB100-55391) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Hexim 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HRQQERAPLSKFGD corresponding to C-Terminus according to NP_006451.1.
• Epitope: HRQQERAPLSKFGD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HEXIM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/mL
• Control:

  Hexim 1 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Hexim 1 Antibody

• Cardiac lineage protein 1
• CLP1
• CLP-1
• CLP1hexamethylene-bis-acetamide-inducible transcript 1
• EDG1HMBA-inducible
• Estrogen down-regulated gene 1 protein
• hexamethylene bis-acetamide inducible 1
• Hexamethylene bis-acetamide-inducible protein 1
• hexamthylene bis-acetamide inducible 1
• Hexim 1
• HEXIM1
• HIS1
• HIS1FLJ13562
• HMBA-inducible
• MAQ1
• MAQ1hexamethylene bisacetamide-inducible protein
• menage a quatre 1
• Menage a quatre protein 1
• protein HEXIM1

Background

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hexim 1 Antibody (NB100-55391)(1)

Publication using NB100-55391

• Schulte A, Czudnochowski N, Barboric M et al. Identification of a Cyclin T-Binding Domain in Hexim1 and Biochemical Analysis of Its Binding Competition with HIV-1 Tat. J Biol Chem 2005-07-01 [PMID: 15855166]

Bioinformatics

• Gene Symbol: HEXIM1
• Entrez:
  • 10614(Human)
  • 192231(Mouse)
• Uniprot:
  • NP_006451.1(Human)