Hexim 1 Overexpression Lysate

Images

 
Western Blot: HEXIM1 Overexpression Lysate (Adult Normal) [NBL1-11518] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HEXIM1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Hexim 1 Overexpression Lysate Summary

Description
Transient overexpression lysate of hexamethylene bis-acetamide inducible 1 (HEXIM1) The lysate was created in HEK293T cells, using Plasmid ID RC200536 and based on accession number NM_006460. The protein contains a C-MYC/DDK Tag.
Gene
HEXIM1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Hexim 1 Overexpression Lysate

  • Cardiac lineage protein 1
  • CLP1
  • CLP-1
  • CLP1hexamethylene-bis-acetamide-inducible transcript 1
  • EDG1HMBA-inducible
  • Estrogen down-regulated gene 1 protein
  • hexamethylene bis-acetamide inducible 1
  • Hexamethylene bis-acetamide-inducible protein 1
  • hexamthylene bis-acetamide inducible 1
  • Hexim 1
  • HEXIM1
  • HIS1
  • HIS1FLJ13562
  • HMBA-inducible
  • MAQ1
  • MAQ1hexamethylene bisacetamide-inducible protein
  • menage a quatre 1
  • Menage a quatre protein 1
  • protein HEXIM1

Background

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67811
Species: Hu, Mu
Applications: ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP3-05509
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-26691
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-05160
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00002691-T01
Species: Hu
Applications: WB
NBP2-46349
Species: Hu
Applications: IHC, WB
NBP2-24762
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-03363
Species: Ch, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
MAB7089
Species: Mu
Applications: CyTOF-ready, Flow, IHC
NBP2-48990
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-79286
Species: Rt
Applications: WB
MAB4457
Species: Hu, Mu, Rt
Applications: WB
NBP1-93877
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-76393
Species: Hu, Mu
Applications: CHIP-SEQ, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-37477
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-84010
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-15497
Species: Hu, Mu, Rt
Applications: ICC/IF, WB

Publications for Hexim 1 Lysate (NBL1-11518) (0)

There are no publications for Hexim 1 Lysate (NBL1-11518).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Hexim 1 Lysate (NBL1-11518) (0)

There are no reviews for Hexim 1 Lysate (NBL1-11518). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Hexim 1 Lysate (NBL1-11518) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Hexim 1 Products

Bioinformatics Tool for Hexim 1 Lysate (NBL1-11518)

Discover related pathways, diseases and genes to Hexim 1 Lysate (NBL1-11518). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Hexim 1 Lysate (NBL1-11518)

Discover more about diseases related to Hexim 1 Lysate (NBL1-11518).
 

Pathways for Hexim 1 Lysate (NBL1-11518)

View related products by pathway.

PTMs for Hexim 1 Lysate (NBL1-11518)

Learn more about PTMs related to Hexim 1 Lysate (NBL1-11518).
 

Research Areas for Hexim 1 Lysate (NBL1-11518)

Find related products by research area.

Blogs on Hexim 1

There are no specific blogs for Hexim 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Hexim 1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol HEXIM1