HERV Antibody - Azide and BSA Free

Western Blot: HERV Antibody - Azide and BSA Free [HERV] - Western blot analysis of lysates from BeWo cells, using HERV Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HERV Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 201-300 of human HERV (NP_055405.3). PEQWNNFSTEINTTS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERVW-1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 59 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for HERV Antibody - Azide and BSA Free

• endogenous retroviral family W, env(C7), member 1
• ENV
• envelope glycoprotein
• Envelope polyprotein gPr73
• enverin
• ENVW
• Env-W
• HERV-7q Envelope protein
• HERV7Q
• HERV-7q
• HERV-tryptophan envelope protein
• HERV-W Env glycoprotein
• HERV-W envelope protein
• HERV-W
• HERV-W_7q21.2 provirus ancestral Env polyprotein
• HERV-W{7q21.1} provirus ancestral Env polyprotein
• HERVWENV
• HERV-W-ENV
• HERVWenvelope protein
• human endogenous retrovirus W envC7-1 envelope protein
• syncytin A
• Syncytin
• syncytin-1

Background

HERVs are a class of multiple sclerosis associated retroviruses. Reacts with a replication competent Human Endogenous Retrovirus (HERVW) isolated from placenta. The synthetic peptide sequence used as immunogen is taken from the virus envelope polyprotein sequence. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. The HERVW endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERVW-1