HERC2 Antibody

Images

 
Western Blot: HERC2 Antibody [NBP1-05970] - Detection of Mouse HERC2- Whole cell lysate (50 ug) from NIH3T3, TCMK-1, Renca, 4T1, and CT26.WT cells. Affinity purified rabbit anti-HERC2 antibody used for WB at 0.5 ug/ml. ...read more
Immunoprecipitation: HERC2 Antibody [NBP1-05970] - Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-HERC2 antibody used for WB. HERC2 was also immunoprecipitated by another rabbit anti-HERC2 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

HERC2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 4784 and 4834 of human hect domain and RLD 2 using the numbering given in entry NP_004658.3 (GeneID 8924).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HERC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 1:10-1:500
Theoretical MW
527 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HERC2 Antibody

  • D15F37S1
  • DKFZp547P028
  • E3 ubiquitin-protein ligase HERC2
  • EC 6.3.2
  • EC 6.3.2.-
  • HECT domain and RCC1-like domain-containing protein 2
  • hect domain and RLD 2
  • jdf2
  • KIAA0393
  • p528
  • probable E3 ubiquitin-protein ligase HERC2
  • SHEP1

Background

HERC2 (HECT domain and RCC1-like domain-containing protein 2) belongs to the HERC family of ubiquitin ligases. A single nucleotide polymorphism (SNP) in an evolutionarily conserved region of the HERC2 gene is associated with blue eye color. Additionally, it has been reported that gene-gene interactions between HERC2, OCA2, and MC1R may influence eye, skin, and hair color.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HERC2
Entrez
Uniprot