HERC1 Antibody - BSA Free

Immunoprecipitation: HERC1 Antibody [NBP1-05968] - Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-HERC1 antibody used for IP at 3 mcg/mg lysate.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HERC1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HERC1 Antibody - BSA Free (NBP1-05968) is a polyclonal antibody validated for use in IP. Anti-HERC1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 1 using the numbering given in entry NP_003913.3 (GeneID 892
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HERC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 5 ug/mg lysate
• Application Notes: This antibody is not recommended for Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HERC1 Antibody - BSA Free

• EC 6.3.2
• guanine nucleotide exchange factor p532
• hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1(CHC1)-like domain (RLD) 1
• hect domain and RCC1-like domain 1
• HECT domain and RCC1-like domain-containing protein 1
• p532probable E3 ubiquitin-protein ligase HERC1
• p619EC 6.3.2.-

Background

HERC1 (HECT domain and RCC1-like domain-containing protein 1) belongs to the HERC family of ubiquitin ligases. It is involved in membrane traffic via its ability to bind clathrin and its guanine nucleotide exchange factor (GEF) activity for Arf and Rab. HERC1 has been found to interact with TSC2 (tuberous sclerosis complex 2) and may be involved with its destabilization. Alternative names for HERC1 include p532 and p619.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HERC1 Antibody (NBP1-05968)(1)

Publication using NBP1-05968

• Kang TH, Reardon JT, Sancar A et al. Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein. Nucleic Acids Res 2011-04-01 [PMID: 21193487]

Bioinformatics

• Gene Symbol: HERC1
• Entrez:
  • 8925(Human)
• Uniprot:
  • NP_003913.3(Human)