HERC1 Antibody - BSA Free

Western Blot: HERC1 Antibody [NBP1-05969] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: HERC1 Antibody [NBP1-05969] - Detection of human HERC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC1 antibody NBP1-05969 used for IP at 3 ug per reaction. HERC1 was also immunoprecipitated by rabbit anti-HERC1 antibody NBP1-05968. For blotting immunoprecipitated HERC1, NBP1-05969 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HERC1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HERC1 Antibody - BSA Free (NBP1-05969) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 2400 and 2450 of human hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 1 using the numbering given in entry NP_003913.3 (GeneID 8
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HERC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HERC1 Antibody - BSA Free

• EC 6.3.2
• guanine nucleotide exchange factor p532
• hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1(CHC1)-like domain (RLD) 1
• hect domain and RCC1-like domain 1
• HECT domain and RCC1-like domain-containing protein 1
• p532probable E3 ubiquitin-protein ligase HERC1
• p619EC 6.3.2.-

Background

HERC1 (HECT domain and RCC1-like domain-containing protein 1) belongs to the HERC family of ubiquitin ligases. It is involved in membrane traffic via its ability to bind clathrin and its guanine nucleotide exchange factor (GEF) activity for Arf and Rab. HERC1 has been found to interact with TSC2 (tuberous sclerosis complex 2) and may be involved with its destabilization. Alternative names for HERC1 include p532 and p619. We use the same protocol that we detail on our website (https://www.fortislife.com/products/protocols/western-blot-protocol/protocol022) and in the figure legend. To your specific point about the larger protein, we use a 3-8% tris-acetate SDS-PAGE gel and transfer for 15 minutes on a Biorad quick transfer system. If using a more traditional submersed tank technique, we would transfer overnight at 4C We use a semi-dry PowerBlotter system for our transfers. For most proteins, we do a 8 minute transfer, but for HERC1 (and other larger proteins), we do a 15 minute transfer. Note that for a single gel, the current is 1.3 amps. For two, that increases to 2.5 amps. Also note that the protein must be electrophoresed on a 3-8% tris-acetate gel

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HERC1
• Entrez:
  • 8925(Human)
• Uniprot:
  • NP_003913.3(Human)