Hepassocin/FGL1 Antibody [Unconjugated]

HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules." > AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules." title="HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF1614, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules." />

HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF1614, filled histogram) or control antibody (Catalog # <a class=

Simple Western lane view shows lysates of Hep3B human hepatocellular carcinoma cell line, human liver, loaded at 0.2 mg/mL. A specific band was detected for Hepassocin/FGL1 at approximately 41 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

HAF017). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." > HAF017). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." title="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." />

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Hepassocin/FGL1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
  Met1-Ile312
  Accession # BAB70690
• Specificity: Detects human Hepassocin/FGL1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hepassocin/FGL1 Antibody [Unconjugated]

• FGL1
• fibrinogen-like 1
• fibrinogen-like protein 1
• Fibrinogen-like-protein 1
• FREP1
• Hepassocin
• hepatocellular carcinoma-related sequence
• Hepatocyte-derived fibrinogen-related protein 1
• HFREP-1
• HFREP1MGC12455
• HP-041
• LFIRE1
• LFIRE-1
• Liver fibrinogen-related protein 1

Background

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein (HFREP-1), is a liver specific secreted protein containing one fibrinogen C-terminal domain. It exists as a homodimer and is reported to have hepatocyte mitogenic activity (1).

1. 1.Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepassocin/FGL1 Antibody (AF1614)(1)

Publication using AF1614

• Rijken DC, Dirkx SP, Luider TM, Leebeek FW Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot. Biochem. Biophys. Res. Commun., 2006-09-15;350(1):191-4. 2006-09-15 [PMID: 16996032] (Mass Spectrometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: FGL1
• Uniprot:
  • BAB70690()