Home » Hepassocin/FGL1 » Hepassocin/FGL1 Antibodies » Hepassocin/FGL1 Antibody [Unconjugated]

Hepassocin/FGL1 Antibody [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and H4‑II‑E‑C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF1614, filled histogram) or control antibody (Catalog # ...read more
Simple Western lane view shows lysates of Hep3B human hepatocellular carcinoma cell line, human liver, loaded at 0.2 mg/mL. A specific band was detected for Hepassocin/FGL1 at approximately 41 kDa (as indicated) using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1614
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1614-SP
100% Guarantee on every product

Hepassocin/FGL1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690
Specificity
Detects human Hepassocin/FGL1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FGL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF1614 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hepassocin/FGL1 Antibody [Unconjugated]

  • FGL1
  • fibrinogen-like 1
  • fibrinogen-like protein 1
  • Fibrinogen-like-protein 1
  • FREP1
  • Hepassocin
  • hepatocellular carcinoma-related sequence
  • Hepatocyte-derived fibrinogen-related protein 1
  • HFREP-1
  • HFREP1MGC12455
  • HP-041
  • LFIRE1
  • LFIRE-1
  • Liver fibrinogen-related protein 1

Background

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein (HFREP-1), is a liver specific secreted protein containing one fibrinogen C-terminal domain. It exists as a homodimer and is reported to have hepatocyte mitogenic activity (1).
  1. 1.Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hepassocin/FGL1 Antibody (AF1614)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol FGL1
Uniprot