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HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for Hepassocin/FGL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
HAF018). A specific band was detected for Hepassocin at approximately 68 kDa for the dimer and 34 kDa for the monomer (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
Species: Hu Applications: WB, IHC Host: Mouse Monoclonal
2319-L3) is immobilized at 2.50 μg/mL, 100 μL/well, Recombinant Cynomolgus Monkey/Rhesus Macaque Hepassocin/FGL1 Fc Chimera Protein (Catalog # 11176-HE) binds with an ED50 of 0.500-4.00 µg/mL." class="big_lightbox" target="_blank">
2319-L3) is immobilized at 2.50 µg/mL (100 µL/ well), Biotinylated Recombinant Human Hepassocin/FGL1 Avi-tag Fc Chimera (Catalog # AVI10573) binds with an ED50 of 0.250-2.50 μg/mL." class="big_lightbox" target="_blank">
2319-L3) is coated at 1 µg/mL (100 µL/well), the concentration of Human Hepassocin/FGL1 His-tag (Catalog # 10285-HE) that produces 50% of the optimal binding response is 0.05-0.4 µg/mL." class="big_lightbox" target="_blank">