Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody [Unconjugated]

Western blot shows lysates of U‑118‑MG human glioblastoma/astrocytoma cell line, Neuro‑2A mouse neuroblastoma cell line, and SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6085) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti‑Human/Mouse Heparan Sulfate 3‑O-Sulfotransferase 4/HS3ST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6085) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Heparan Sulfate 3-O-Sulfotransferase4/HS3ST4
  Gly184-Lys456
  Accession # Q9Y661
• Specificity: Detects human and mouse Heparan Sulfate 3-O-Sulfotransferase4/HS3ST4 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HS3ST4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody [Unconjugated]

• 30ST4
• 3OST4 heparan sulfate 3-O-sulfotransferase-4
• 3-OST-4
• EC 2.8.2
• EC 2.8.2.23
• h3-OST-4
• heparan sulfate (glucosamine) 3-O-sulfotransferase 4
• Heparan sulfate 3-O-sulfotransferase 4
• Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4
• heparan sulfate glucosamine 3-O-sulfotransferase 4
• HS3ST4

Background

Heparan Sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of Heparan Sulfate that is predominantly found in mast cells. Both heparin and Heparan Sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by Heparan Sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), Heparan Sulfate 3-O sulfotransferases (HS3STs) and Heparan Sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated Heparan Sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5). The enzyme activity was assayed using an SDS-PAGE based method (9).

1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
4. Xu, D. et al. (2005) Biochem. J. 386:451.
5. Lawrence, R. et al. (2007) Matrix Biol. 26:442.
6. Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
7. Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
8. Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.
9. Wu, Z.L. et al. (2010) BMC Biotechnol. 10:11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HS3ST4
• Uniprot:
  • Q9Y661()