Hemoglobin beta Antibody (23E5.H6.G6.C1.H7.F7.G9.F6) - N-terminal

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 23E5.H6.G6.C1.H7.F7.G9.F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Hemoglobin beta Antibody (23E5.H6.G6.C1.H7.F7.G9.F6) - N-terminal Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.
• Specificity: Reacts specifically with human HbS beta sickle isoform. This antibody does not react with the HbA, HbF, HbC, or HbA-2 isoform.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: HBB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: This antibody has been tested by ELISA and western blot. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa in appropriate lysates.
  
  Suggested applications: FC; IF; LFA

Reactivity Notes

Blast analysis shows 100% homology to Human, Pan troglodytes, Pan paniscus, Gorilla gorilla gorilla, and Hylobates lar.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Hemoglobin beta Antibody (23E5.H6.G6.C1.H7.F7.G9.F6) - N-terminal

• beta globin chain
• beta-globin
• CD113t-C
• HBBB
• HBD
• Hemoglobin beta chain
• hemoglobin subunit beta
• hemoglobin, beta

Background

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HBB