HE4/WFDC2 Antibody


Western Blot: HE4/WFDC2 Antibody [NBP1-49888] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human WFDC2 with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
0.5 mg/ml

Order Details

HE4/WFDC2 Antibody Summary

Peptide with sequence GAEKTGVCPELQADQ corresponding to internal region according to NP_006094.3.
GAEKTGVCPELQADQ - internal region
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline, pH 7.3 with 0.5% BSA.
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


Application Notes
WB: In transfected HEK293 transiently expressing full-length human WFDC2 (myc and DYKDDDDK tagged), a band of approx. 25 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
Read Publication using NBP1-49888.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.


Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HE4/WFDC2 Antibody

  • dJ461P17.6
  • EDDM4
  • Epididymal secretory protein E4
  • epididymis-specific, whey-acidic protein type, four-disulfide core
  • HE4MGC57529
  • Major epididymis-specific protein E4
  • Putative protease inhibitor WAP5
  • WAP domain containing protein HE4-V4
  • WAP four-disulfide core domain 2
  • WAP four-disulfide core domain protein 2
  • WAP5
  • WAP5epididymal protein 4
  • WFDC2


This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HE4/WFDC2 Antibody (NBP1-49888)(1)

Reviews for HE4/WFDC2 Antibody (NBP1-49888) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HE4/WFDC2 Antibody (NBP1-49888). (Showing 1 - 1 of 1 FAQ).

  1. I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
    • At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following link: www.novusbio.com/support/innovators-reward.html

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol WFDC2

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