HE4/WFDC2 Antibody

Images

 
Western Blot: HE4/WFDC2 Antibody [NBP1-49888] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human WFDC2 with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HE4/WFDC2 Antibody Summary

Immunogen
Peptide with sequence GAEKTGVCPELQADQ corresponding to internal region according to NP_006094.3.
Epitope
GAEKTGVCPELQADQ - internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WFDC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: In transfected HEK293 transiently expressing full-length human WFDC2 (myc and DYKDDDDK tagged), a band of approx. 25 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
Publications
Read Publication using NBP1-49888.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HE4/WFDC2 Antibody

  • dJ461P17.6
  • EDDM4
  • Epididymal secretory protein E4
  • epididymis-specific, whey-acidic protein type, four-disulfide core
  • HE4
  • HE4MGC57529
  • Major epididymis-specific protein E4
  • Putative protease inhibitor WAP5
  • WAP domain containing protein HE4-V4
  • WAP four-disulfide core domain 2
  • WAP four-disulfide core domain protein 2
  • WAP5
  • WAP5epididymal protein 4
  • WFDC2

Background

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HE4/WFDC2 Antibody (NBP1-49888)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for HE4/WFDC2 Antibody (NBP1-49888). (Showing 1 - 1 of 1 FAQ).

  1. I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
    • At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following link: www.novusbio.com/support/innovators-reward.html

Secondary Antibodies

 

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Diseases for HE4/WFDC2 Antibody (NBP1-49888)

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Bioinformatics

Gene Symbol WFDC2
Entrez
Uniprot