HE4/WFDC2 Antibody (676013) [Unconjugated]

HE4/WFDC2 was detected in immersion fixed paraffin-embedded sections of human ovary using Mouse Anti-Human HE4/WFDC2 Monoclonal Antibody (Catalog # MAB6274) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 676013
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HE4/WFDC2 Antibody (676013) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HE4/WFDC2
  Thr28-Phe124
  Accession # Q14508
• Specificity: Detects human HE4/WFDC2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: WFDC2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HE4/WFDC2 Antibody (676013) [Unconjugated]

• dJ461P17.6
• EDDM4
• Epididymal secretory protein E4
• epididymis-specific, whey-acidic protein type, four-disulfide core
• HE4
• HE4MGC57529
• Major epididymis-specific protein E4
• Putative protease inhibitor WAP5
• WAP domain containing protein HE4-V4
• WAP four-disulfide core domain 2
• WAP four-disulfide core domain protein 2
• WAP5
• WAP5epididymal protein 4
• WFDC2

Background

HE4 (also WFDC2; WAP Four-disulfide core domain protein 2) is a secreted, 25 kDa glycoprotein member of the Whey Acidic Protein family of molecules. It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium. It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase). Ovarian epithelial tumors have an increased expression of HE4, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer. Mature human HE4 is 94 amino acids (aa) in length. It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa 31‑73 and 74‑123). There are four potential splice variants. One shows a deletion of aa 27‑74, while three others show aa substitutions: 28 aa for aa 75‑124, 23 aa for aa 1‑74, and 10 aa for aa 71‑124. Over aa 31‑124, human HE4 shares 63% aa identity with an extended (144 amino acid) mouse form of mature HE4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HE4/WFDC2 Antibody (MAB6274)(1)

Publication using MAB6274

• Alba Galan, Arturo Papaluca, Ali Nejatie, Emad Matanes, Fouad Brahimi, Wenyong Tong et al. GD2 and GD3 gangliosides as diagnostic biomarkers for all stages and subtypes of epithelial ovarian cancer Frontiers in Oncology 2023-04-14 [PMID: 37124505]

FAQs for HE4/WFDC2 Antibody (MAB6274). (Showing 1 - 1 of 1 FAQ).

1. I would like to build a sandwich ELISA for detection of HE4 levels in human blood. I found there are several HE4 antibodies from your company. Could you please provide me some information among these products, which two antibodies will work well with the HE4 antigen in a sandwich ELISA pair?
   • At this time we have not tested any of our HE4 antibodies for use in a Sandwich ELISA. As such, we cannot say with certainty, which will work together as an effective pair. Often times in our experience, choosing a monoclonal antibody for capture, and a polyclonal antibody for detection will yield great results. If you plan on testing any of our HE4 antibodies for use in a Sandwich ELISA then we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results. Additional Innovator’s Reward information can be found at the following here

Bioinformatics

• Gene Symbol: WFDC2
• Uniprot:
  • Q14508(Human)