HDGF Antibody (PCRP-HDGF-1D1)

Flow cytometric analysis of PFA-fixed HeLa cells. HDGF Antibody (PCRP-HDGF-1D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Formalin-fixed, paraffin-embedded human colon carcinoma stained with HDGF Antibody (PCRP-HDGF-1D1). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Analysis of Protein Array containing more than 19,000 full-length human proteins using HDGF Antibody (PCRP-HDGF-1D1).

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: PCRP-HDGF-1D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

HDGF Antibody (PCRP-HDGF-1D1) Summary

• Description: Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant full-length human HDGF protein
• Localization: Nucleus, Cytoplasm
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HDGF
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2ug/million cells
  • Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT
• Application Notes: Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for HDGF Antibody (PCRP-HDGF-1D1)

• FLJ96580
• HDGF
• hepatoma-derived growth factor (high-mobility group protein 1-like)
• hepatoma-derived growth factor
• High mobility group protein 1-like 2
• HMG-1L2
• HMG1L2DKFZp686J1764
• HuHGF

Background

The HDGF gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic andDNA-binding activity and may play a role in cellular proliferation and differentiation. This gene was thoughtinitially to be located on chromosom

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDGF