Recombinant Human HDAC3 Protein

Product Discontinued

Recombinant Human HDAC3 Protein Summary

• Description: Recombinant protein for Human HDAC3
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(O15379)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: HDAC3
• Purity: >50% SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Active HDAC3 is useful in studying enzyme regulation, determining target substrates, screening deacetylase inhibitors, or as a positive control in HDAC activity assays. We recommend using 1- 5 unit/assay for analyzing HDAC3 activity.
  Each Definition: One unit of the recombinant HDAC3 is defined as the amount of enzyme that deacetylates 1 pmol of substrate Arg-His-Lys-Lys(Ac)-AFC per minute at 37C in a reaction solution containing 50 mM Tris pH 7.5 137 mM NaCl 2.7 mM KCl 1 mM MgCl2 and 1 BSA.
  Specific activity: Activity of rh-HDAC3 was tested using Arg-His-Lys-Lys(Ac)-AFC, a fluorogenic, acetylated peptide based on residues 379-382 of p53 as a substrate. > 0.5 U/ul
• Theoretical MW: 51.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 25 mM Tris (pH 7.5), 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2, 10 % glycerol
• Concentration: 0.5 mg/ml
• Purity: >50% SDS-PAGE

Alternate Names for Recombinant Human HDAC3 Protein

• EC 3.5.1.98
• HD3RPD3-2RPD3
• HDAC3
• Histone Deacetylase 3
• KDAC3
• RPD3-2
• SMAP45

Background

Human Histone Deacetylase 3 (HDAC3) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Like other class I and II HDAC members, the activity of HDAC3 is sensitive to HDAC inhibitor Trichostatin A (TSA).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HDAC3