HDAC3 Antibody (SC55-02)

Western Blot: HDAC3 Antibody (SC55-02) [NBP2-67592] - Western blot analysis of HDAC3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody (SC55-02) [NBP2-67592] - Staining HDAC3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HDAC3 Antibody (SC55-02) [NBP2-67592] - Analysis of paraffin-embedded mouse skin tissue using anti-HDAC3 antibody. Counter stained with hematoxylin.

Western Blot: HDAC3 Antibody (SC55-02) [NBP2-67592] - Analysis of HDAC3 on NIH/3T3 cell lysates using anti-HDAC3 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody (SC55-02) [NBP2-67592] - Staining HDAC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HDAC3 Antibody (SC55-02) [NBP2-67592] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-HDAC3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HDAC3 Antibody (SC55-02) [NBP2-67592] - Analysis of paraffin-embedded human tonsil tissue using anti-HDAC3 antibody. Counter stained with hematoxylin.

Product Discontinued

HDAC3 Antibody (SC55-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human HDAC3. (SwissProt: O15379 Human; SwissProt: O88895 Mouse; SwissProt: Q6P6W3 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HDAC3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HDAC3 Antibody (SC55-02)

• EC 3.5.1.98
• HD3RPD3-2RPD3
• HDAC3
• Histone Deacetylase 3
• KDAC3
• RPD3-2
• SMAP45

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC3