HCN4 Antibody [Unconjugated]

HCN4 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into cardiomyocytes (upper panel), using Sheep Anti-Human HCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8138) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

HCN4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HCN4
  Lys1084-Leu1203
  Accession # Q9Y3Q4
• Specificity: Detects human HCN4 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HCN4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HCN4 Antibody [Unconjugated]

• HCN4
• hyperpolarization activated cyclic nucleotide-gated cation channel 4
• hyperpolarization activated cyclic nucleotide-gated potassium channel 4
• potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
• SSS2

Background

HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) is a 120-160 kDa, 1203 amino acid (aa) multipass plasma membrane protein that is a member of the HCN family of potassium channel proteins. It is mainly expressed in cardiac myocytes, thalamus and testis. HCN4 is important for pacemaker function in the heart, and rhythmic activity in the thalamus. Specific mutations can cause Sick Sinus Syndrome 2 (SSS2), a cardiac sinus node malfunction, or Brugada syndrome 8 (BRGDA8), a tachyarrhythmia syndrome. Within aa 1084-1203, human HCN4 shares 92% and 93% aa sequence identity with mouse and rat HCN4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HCN4
• Uniprot:
  • Q9Y3Q4()