Recombinant Human Hck Protein

Product Discontinued

Recombinant Human Hck Protein Summary

• Description: Recombinant protein for Human Hck
  
  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(PO8631)
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab60865)
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: HCK
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Mass Spectrometry
  • SDS-Page
• Application Notes: Use in MS reported in scientific literature (PMID:34320188). Specific activity: 171 nmol phosphate incorporated into poly(Glu-Tyr). per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay)..
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Hck Protein

• Bmk
• EC 2.7.10
• EC 2.7.10.2
• Hck
• Hctk
• hemopoietic cell kinaseJTK9
• JTK9
• p59-HCK/p60-HCK
• tyrosine-protein kinase HCK

Background

HCK, a protein-tyrosine kinase, belongs to SRC family members (1). Ziegler et al. found that expression of HCK may be limited to certain hemopoietic cells and is especially prominent in cells of myeloid lineage, particularly mature granulocytes and monocytes (2). Therefore, Quintrell et al. designated the gene HCK (pronounced 'hick') for hemopoietic cell kinase. They described the nucleotide sequence of a cDNA clone and the distribution of RNA transcribed from HCK among various hemopoietic cells. They assigned the HCK gene to 20q11-q12. Since this region is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders, they suggested that damage to HCK may contribute to the pathogenesis of these conditions (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Hck Recombinant Protein (NBP1-99500)(1)

Publication using NBP1-99500

• Brian Iv Bf, Freedman Ts The Src-family kinase Lyn in immunoreceptor signaling Endocrinology 2021-07-28 [PMID: 34320188] (MS)

Bioinformatics

• Gene Symbol: HCK