Recombinant Human Hck Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity, MS
Concentration
0.1 mg/ml

Order Details

Recombinant Human Hck Protein Summary

Description
Recombinant protein for Human Hck

Source:Baculovirus (Sf9 insect cells)
Amino Acid Sequence:(PO8631)

Source
Sf 9 (baculovirus)
Protein/Peptide Type
Recombinant Protein
Gene
HCK
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Bioactivity
  • Mass Spectrometry
  • SDS-Page
Application Notes
Use in MS reported in scientific literature (PMID:34320188). Specific activity: 171 nmol phosphate incorporated into poly(Glu-Tyr). per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay)..
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-99500.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Concentration
0.1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Hck Protein

  • Bmk
  • EC 2.7.10
  • EC 2.7.10.2
  • Hck
  • Hctk
  • hemopoietic cell kinaseJTK9
  • JTK9
  • p59-HCK/p60-HCK
  • tyrosine-protein kinase HCK

Background

HCK, a protein-tyrosine kinase, belongs to SRC family members (1). Ziegler et al. found that expression of HCK may be limited to certain hemopoietic cells and is especially prominent in cells of myeloid lineage, particularly mature granulocytes and monocytes (2). Therefore, Quintrell et al. designated the gene HCK (pronounced 'hick') for hemopoietic cell kinase. They described the nucleotide sequence of a cDNA clone and the distribution of RNA transcribed from HCK among various hemopoietic cells. They assigned the HCK gene to 20q11-q12. Since this region is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders, they suggested that damage to HCK may contribute to the pathogenesis of these conditions (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Hck Recombinant Protein (NBP1-99500)(1)

We have publications tested in 1 application: MS.


Filter By Application
MS
(1)
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Bioinformatics

Gene Symbol HCK