Hck Overexpression Lysate


Western Blot: Hck Overexpression Lysate (Adult Normal) [NBP2-08146] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hck.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

Hck Overexpression Lysate Summary

The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_002101. The protein contains a C-terminal DDK tag.
Homo sapiens Hck, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
59.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Hck Overexpression Lysate

  • Bmk
  • EC 2.7.10
  • EC
  • Hck
  • Hctk
  • hemopoietic cell kinaseJTK9
  • JTK9
  • p59-HCK/p60-HCK
  • tyrosine-protein kinase HCK


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Simple Western, IHC
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, Simple Western
Species: Hu
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu, Po
Applications: Flow, IHC-Fr, IF
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB

Publications for Hck Lysate (NBP2-08146) (0)

There are no publications for Hck Lysate (NBP2-08146).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Hck Lysate (NBP2-08146) (0)

There are no reviews for Hck Lysate (NBP2-08146). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Hck Lysate (NBP2-08146) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats


Additional Hck Products

Bioinformatics Tool for Hck Lysate (NBP2-08146)

Discover related pathways, diseases and genes to Hck Lysate (NBP2-08146). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Hck Lysate (NBP2-08146)

Discover more about diseases related to Hck Lysate (NBP2-08146).

Pathways for Hck Lysate (NBP2-08146)

View related products by pathway.

PTMs for Hck Lysate (NBP2-08146)

Learn more about PTMs related to Hck Lysate (NBP2-08146).

Research Areas for Hck Lysate (NBP2-08146)

Find related products by research area.

Blogs on Hck

There are no specific blogs for Hck, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Hck Overexpression Lysate and receive a gift card or discount.


Gene Symbol HCK