HAX-1 Overexpression Lysate (Native)

Images

 
Western Blot: HAX1 Overexpression Lysate (Adult Normal) [NBL1-11453] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HAX1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HAX-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202690 and based on accession number NM_006118. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens HCLS1 associated protein X-1 (HAX1), transcript variant 1, mRNA.
Gene
HAX1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HAX-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HAX-1 Overexpression Lysate (Native)

  • FLJ17042
  • FLJ93803
  • HAX1
  • HAX-1
  • HCLS1 (and PKD2) associated protein
  • HCLS1 associated protein X-1
  • HCLS1-associated protein X-1
  • HCLSBP1
  • HS1 binding protein
  • HS1-associating protein X-1
  • HS1-binding protein 1
  • HS1BP1
  • HS1BP1FLJ18492
  • HSP1BP-1
  • SCN3

Background

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HAX-1 Lysate (NBL1-11453) (0)

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Bioinformatics

Gene Symbol HAX1
Entrez