HAUS8 Antibody - BSA Free

Western Blot: HAUS8 Antibody [NBP1-77746] - Analysis shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HAUS8. In right lane (HAUS8): lysates from sh-HAUS8 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HAUS8 Antibody - BSA Free Summary

• Description: This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: HAUS8 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length HAUS8 protein. (Uniprot: Q9BT25)
• Specificity: This antibody reacts with endogenous HAUS8 protein.
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HAUS8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:250000
  • Western Blot 1:10000
• Application Notes: This antiserum has been tested for use in ELISA and western blotting using a full length recombinant Hice1 protein. Specific conditions for reactivity and detection of Hice1 should be optimized by the end user. Expect a band approximately ~45 kDa in size corresponding to Hice1 by Western Blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest reactivity with HAUS8 from human based on a 100% homology with the immunizing sequence. Expect reactivity with HAUS8 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with HAUS8 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HAUS8 Antibody - BSA Free

• DGT4
• HAUS Augmin Like Complex Subunit 8
• HEC1/NDC80 interacting, centrosome associated 1
• HEC1/NDC80-Interacting Centrosome-Associated Protein 1
• Hec1-Interacting And Centrosome-Associated 1
• HICE1
• NY-SAR-48
• Sarcoma Antigen NY-SAR-48

Background

HAUS8 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the 'TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAUS8
• Uniprot:
  • NP_219485(Human)
  • Q9BT25(Human)