HAI-2/SPINT2 Antibody

Western Blot: HAI-2/SPINT2 Antibody [NBP2-26109] - Staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HAI-2/SPINT2 Antibody Summary

• Description: Novus Biologicals Goat HAI-2/SPINT2 Antibody (NBP2-26109) is a polyclonal antibody validated for use in WB and ELISA. Anti-HAI-2/SPINT2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SGDDKEQLVKNT corresponding to C-Terminus according to NP_001159575.1.
• Specificity: This antibody is expected to recognize to recognize both reported isoforms (NP_066925.1; NP_001159575.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPINT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 20 kDa band observed in human placenta lysates (calculated MW of 21.8 kDa band according to NP_001159575.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HAI-2/SPINT2 Antibody

• HAI2
• HAI-2
• HAI-2DIAR3
• HAI2FLJ45571
• Hepatocyte growth factor activator inhibitor type 2
• Kop
• kunitz-type protease inhibitor 2
• PB
• Placental bikunin
• serine peptidase inhibitor, Kunitz type, 2
• serine protease inhibitor, Kunitz type, 2

Background

The SPINT2 gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This gene is a putative tumor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAI-2/SPINT2 Antibody (NBP2-26109)(1)

Publication using NBP2-26109

• Sivagnanam M, Janecke AR, Muller T et al. Case of syndromic tufting enteropathy harbors SPINT2 mutation seen in congenital sodium diarrhea. Clin Dysmorphol 2010-01-01 [PMID: 20009592]

Bioinformatics

• Gene Symbol: SPINT2
• Entrez:
  • 10653(Human)
  • 20733(Mouse)
  • 292770(Rat)