HAI-1/HGFA Inhibitor 1 Antibody

• Reactivity: Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HAI-1/HGFA Inhibitor 1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
  Glu30-Glu443 (Cys325Ser)
  Accession # NP_058603
• Specificity: Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPINT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-1/HGFA Inhibitor 1 Antibody

• HAI
• HAI1
• HAI-1
• hepatocyte growth factor activator inhibitor 1
• Hepatocyte growth factor activator inhibitor type 1
• kunitz-type protease inhibitor 1
• MANSC2
• serine peptidase inhibitor, Kunitz type 1
• serine protease inhibitor, Kunitz type 1
• SPINT1

Background

Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta . The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

1. Denda, et al. (2002) J. Biol. Chem. 277:14053.
2. Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
3. Kataoka, et al. (2000) J. Biol. Chem. 275:40453.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAI-1/HGFA Inhibitor 1 Antibody (AF1141)(9)

Publications using AF1141

• S Ozawa, M Matsubayas, H Nanaura, M Yanagita, K Mori, K Asanuma, N Kajiwara, K Hayashi, H Ohashi, M Kasahara, H Yokoi, H Kataoka, E Mori, T Nakagawa Proteolytic Cleavage of Podocin by Matriptase Exacerbates Podocyte Injury J. Biol. Chem., 2020-09-09;0(0):. 2020-09-09 [PMID: 32907879] (IHC, Western Blot, Human, Mouse)
• CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020-04-21;9(4):. 2020-04-21 [PMID: 32326212] (Western Blot, Mouse)
• Mikelis C, Palmby T, Simaan M, Li W, Szabo R, Lyons R, Martin D, Yagi H, Fukuhara S, Chikumi H, Galisteo R, Mukouyama Y, Bugge T, Gutkind J PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation. J Biol Chem, 2013-03-06;288(17):12232-43. 2013-03-06 [PMID: 23467409] (IHC-P, Mouse)
• Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis. Cancer Res, 2013-02-27;73(8):2659-70. 2013-02-27 [PMID: 23447577] (IHC, Western Blot, Mouse)
• Szabo , Roman, Uzzun Sales , Katiuchi, Kosa , Peter, Shylo , Natalia, Godiksen , Sine, Hansen , Karina K, Friis , Stine, Gutkind , J Silvio, Vogel , Lotte K, Hummler , Edith, Camerer , Eric, Bugge , Thomas H Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation. PLoS Genet, 2012-08-30;8(8):e1002937. 2012-08-30 [PMID: 22952456] (IHC-P, Immunoprecipitation, Mouse)
• Phin S, Marchand-Adam S, Fabre A, Marchal-Somme J, Bantsimba-Malanda C, Kataoka H, Soler P, Crestani B Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice. Am. J. Respir. Cell Mol. Biol., 2009-05-15;42(3):286-93. 2009-05-15 [PMID: 19448157] (IHC-P, Neutralization, Mouse)
• Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity. J. Biol. Chem., 2008-08-19;283(43):29495-504. 2008-08-19 [PMID: 18713750] (IHC-P, Mouse)
• Godiksen S, Selzer-Plon J, Pedersen ED, Abell K, Rasmussen HB, Szabo R, Bugge TH, Vogel LK Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary. Biochem. J., 2008-07-15;413(2):251-9. 2008-07-15 [PMID: 18402552] (ICC, Canine)
• Szabo R, Molinolo A, List K, Bugge TH Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development. Oncogene, 2006-09-18;26(11):1546-56. 2006-09-18 [PMID: 16983341] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: SPINT1
• Uniprot:
  • NP_058603()