HAI-1/HGFA Inhibitor 1 Antibody

Images

 
There are currently no images for HAI-1/HGFA Inhibitor 1 Antibody (AF1141).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

HAI-1/HGFA Inhibitor 1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Glu30-Glu443 (Cys325Ser)
Accession # NP_058603
Specificity
Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPINT1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publications using
AF1141 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-1/HGFA Inhibitor 1 Antibody

  • HAI
  • HAI1
  • HAI-1
  • hepatocyte growth factor activator inhibitor 1
  • Hepatocyte growth factor activator inhibitor type 1
  • kunitz-type protease inhibitor 1
  • MANSC2
  • serine peptidase inhibitor, Kunitz type 1
  • serine protease inhibitor, Kunitz type 1
  • SPINT1

Background

Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

  1. Denda, et al. (2002) J. Biol. Chem. 277:14053.
  2. Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, et al. (2000) J. Biol. Chem. 275:40453.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3946
Species: Hu
Applications: WB, CyTOF-ready, ICFlow
AF1715
Species: Mu
Applications: WB, IHC, IP
AF276
Species: Hu
Applications: WB, Flow, IHC, Block, CyTOF-ready, ICC, KO
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00005241-M08
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF4599
Species: Hu
Applications: WB, Simple Western, IHC, IP
4776-SE
NBP2-94657
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF1141
Species: Mu
Applications: WB, IHC, IP

Publications for HAI-1/HGFA Inhibitor 1 Antibody (AF1141)(8)

We have publications tested in 2 confirmed species: Mouse, Canine.

We have publications tested in 5 applications: ICC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
ICC
(1)
IHC-P
(3)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(7)
Canine
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1141 Applications Species
CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020;9(4):. 2020 [PMID: 32326212] (Western Blot, Mouse) Western Blot Mouse
Mikelis C, Palmby T, Simaan M, Li W, Szabo R, Lyons R, Martin D, Yagi H, Fukuhara S, Chikumi H, Galisteo R, Mukouyama Y, Bugge T, Gutkind J PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation. J Biol Chem, 2013;288(17):12232-43. 2013 [PMID: 23467409] (IHC-P, Mouse) IHC-P Mouse
Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis. Cancer Res, 2013;73(8):2659-70. 2013 [PMID: 23447577] (Western Blot, Mouse) Western Blot Mouse
Szabo, Roman, Uzzun Sales, Katiuchi, Kosa, Peter, Shylo, Natalia, Godiksen, Sine, Hansen, Karina K, Friis, Stine, Gutkind, J Silvio, Vogel, Lotte K, Hummler, Edith, Camerer, Eric, Bugge, Thomas H Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation. PLoS Genet, 2012;8(8):e1002937. 2012 [PMID: 22952456] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Phin S, Marchand-Adam S, Fabre A, Marchal-Somme J, Bantsimba-Malanda C, Kataoka H, Soler P, Crestani B Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice. Am. J. Respir. Cell Mol. Biol., 2010;42(3):286-93. 2010 [PMID: 19448157] (Neutralization, Mouse) Neutralization Mouse
Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity. J. Biol. Chem., 2008;283(43):29495-504. 2008 [PMID: 18713750] (IHC-P, Mouse) IHC-P Mouse
Godiksen S, Selzer-Plon J, Pedersen ED, Abell K, Rasmussen HB, Szabo R, Bugge TH, Vogel LK Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary. Biochem. J., 2008;413(2):251-9. 2008 [PMID: 18402552] (ICC, Canine) ICC Canine
Szabo R, Molinolo A, List K, Bugge TH Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development. Oncogene, 2006;26(11):1546-56. 2006 [PMID: 16983341] (IHC-P, Mouse) IHC-P Mouse

Reviews for HAI-1/HGFA Inhibitor 1 Antibody (AF1141) (0)

There are no reviews for HAI-1/HGFA Inhibitor 1 Antibody (AF1141). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HAI-1/HGFA Inhibitor 1 Antibody (AF1141) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1141

Additional HAI-1/HGFA Inhibitor 1 Products

Bioinformatics Tool for HAI-1/HGFA Inhibitor 1 Antibody (AF1141)

Discover related pathways, diseases and genes to HAI-1/HGFA Inhibitor 1 Antibody (AF1141). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HAI-1/HGFA Inhibitor 1 Antibody (AF1141)

Discover more about diseases related to HAI-1/HGFA Inhibitor 1 Antibody (AF1141).
 

Pathways for HAI-1/HGFA Inhibitor 1 Antibody (AF1141)

View related products by pathway.

PTMs for HAI-1/HGFA Inhibitor 1 Antibody (AF1141)

Learn more about PTMs related to HAI-1/HGFA Inhibitor 1 Antibody (AF1141).

Blogs on HAI-1/HGFA Inhibitor 1

There are no specific blogs for HAI-1/HGFA Inhibitor 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HAI-1/HGFA Inhibitor 1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SPINT1
Uniprot