HAI-1/HGFA Inhibitor 1 Antibody


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Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC, IP

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HAI-1/HGFA Inhibitor 1 Antibody Summary

Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Glu30-Glu443 (Cys325Ser)
Accession # NP_058603
Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF1141 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAI-1/HGFA Inhibitor 1 Antibody

  • HAI
  • HAI1
  • HAI-1
  • hepatocyte growth factor activator inhibitor 1
  • Hepatocyte growth factor activator inhibitor type 1
  • kunitz-type protease inhibitor 1
  • MANSC2
  • serine peptidase inhibitor, Kunitz type 1
  • serine protease inhibitor, Kunitz type 1
  • SPINT1


Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta . The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

  1. Denda, et al. (2002) J. Biol. Chem. 277:14053.
  2. Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, et al. (2000) J. Biol. Chem. 275:40453.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HAI-1/HGFA Inhibitor 1 Antibody (AF1141)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 5 applications: ICC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.

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Showing Publications 1 - 9 of 9.
Publications using AF1141 Applications Species
S Ozawa, M Matsubayas, H Nanaura, M Yanagita, K Mori, K Asanuma, N Kajiwara, K Hayashi, H Ohashi, M Kasahara, H Yokoi, H Kataoka, E Mori, T Nakagawa Proteolytic Cleavage of Podocin by Matriptase Exacerbates Podocyte Injury J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32907879] (Western Blot, Human) Western Blot Human
CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020;9(4):. 2020 [PMID: 32326212] (Western Blot, Mouse) Western Blot Mouse
Mikelis C, Palmby T, Simaan M, Li W, Szabo R, Lyons R, Martin D, Yagi H, Fukuhara S, Chikumi H, Galisteo R, Mukouyama Y, Bugge T, Gutkind J PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation. J Biol Chem, 2013;288(17):12232-43. 2013 [PMID: 23467409] (IHC-P, Mouse) IHC-P Mouse
Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis. Cancer Res, 2013;73(8):2659-70. 2013 [PMID: 23447577] (Western Blot, Mouse) Western Blot Mouse
Szabo , Roman, Uzzun Sales , Katiuchi, Kosa , Peter, Shylo , Natalia, Godiksen , Sine, Hansen , Karina K, Friis , Stine, Gutkind , J Silvio, Vogel , Lotte K, Hummler , Edith, Camerer , Eric, Bugge , Thomas H Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation. PLoS Genet, 2012;8(8):e1002937. 2012 [PMID: 22952456] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Phin S, Marchand-Adam S, Fabre A, Marchal-Somme J, Bantsimba-Malanda C, Kataoka H, Soler P, Crestani B Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice. Am. J. Respir. Cell Mol. Biol., 2010;42(3):286-93. 2010 [PMID: 19448157] (Neutralization, Mouse) Neutralization Mouse
Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity. J. Biol. Chem., 2008;283(43):29495-504. 2008 [PMID: 18713750] (IHC-P, Mouse) IHC-P Mouse
Godiksen S, Selzer-Plon J, Pedersen ED, Abell K, Rasmussen HB, Szabo R, Bugge TH, Vogel LK Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary. Biochem. J., 2008;413(2):251-9. 2008 [PMID: 18402552] (ICC, Canine) ICC Canine
Szabo R, Molinolo A, List K, Bugge TH Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development. Oncogene, 2006;26(11):1546-56. 2006 [PMID: 16983341] (IHC-P, Mouse) IHC-P Mouse

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Gene Symbol SPINT1