HACE1 Antibody (8D3)

Product Discontinued

HACE1 Antibody (8D3) Summary

• Immunogen: Full length for HACE1 protein was used as the immunogen for this antibody.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HACE1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunofluorescence 1:500
  • Immunohistochemistry 1:800
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Lyophilized antibody can be kept at 4C for up to 3 months and should be kept at -20C for long-term storage. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20C) or for long-term storage (-80C). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with 0.1 ml water.

Alternate Names for HACE1 Antibody (8D3)

• E3 ubiquitin-protein ligase HACE1
• EC 6.3.2
• HACE
• HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
• HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1
• KIAA1320EC 6.3.2.-

Background

The HACE1 gene codes for a E3 ubiquitin-protein ligase HACE1 that is a participant in Golgi membrane fusion as well as in regulation of small GTPases to mediate ubiquitination and degradation of active RAC1, working in defense against pathogens. This protein may also play a role as a transcription regulator through its interaction with RARB. HACE1 exists in four isoforms: isoform 1 is 909 amino acids long, 102 kDA; isoform 2 is 318 amino acids long, 35 kDA; isoform 3 is 562 amino acids long, 63 kDA; isoform 4 is 694 amino acids long, nearly 78 kDA. HACE1 has been researched in regards to celiac disease, malaria, colorectal cancer, wilms tumors, and carcinoma. It is involved in the SMAD signaling network as well as ubiquitin-proteasome dependent proteolysis and has been known to interact with genes RARA, RAB4A, RAC1, PLXNA2, and IRF7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HACE1