HA95/AKAP8L Antibody

Product Discontinued

HA95/AKAP8L Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 596 and 646 of human A kinase (PRKA) anchor protein 8-like (helicase A-binding protein 95 kDa) using the numbering given in entry NP_055186.2 (GeneID 26993).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AKAP8L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  HA95/AKAP8L Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HA95/AKAP8L Antibody

• A kinase (PRKA) anchor protein 8-like
• AKAP8-like protein
• HA95
• HAP95 DKFZp434L0650
• helicase A-binding protein 95 kDa
• Homologous to AKAP95 protein
• HRIHFB2018
• NAKAP
• NAKAP95 A-kinase anchor protein 8-like
• neighbor of A kinase anchoring protein 95
• Neighbor of AKAP95
• Neighbor of A-kinase-anchoring protein 95

Background

AKAP8L/HA95 is a PKA anchoring protein that is localized to the nuclear envelope and associates with chromatin upon nuclear envelope breakdown during mitosis. AKAP8L/HA95 has been found to play an important role in the initiation of DNA replication and may function as a scaffold for proteins such as MCM2 and LAP2beta. Additionally, AKAP8L/HA95 has been shown to co-locate with the PKA C subunit in splicing factor compartments and is involved in the regulation of pre-mRNA splicing. Further support for a scaffolding and splicing role in the nucleus comes from the observation that AKAP8L/HA95 colocalizes with HypA, a splicing-like factor, and through this association may function as a docking site for the nuclear accumulation of Huntington protein as seen in Huntington's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKAP8L
• Entrez:
  • 26993(Human)
• Uniprot:
  • NP_055186.2(Human)