GTF2H2 293T Cell Transient Overexpression Lysate

Product Discontinued

GTF2H2 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-GTF2H2antibody by Western Blot. Plasmid: pCMV-GTF2H2 full-length
• Specificity: GTF2H2 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: GTF2H2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GTF2H2 293T Cell Transient Overexpression Lysate

• Basic transcription factor 2 44 kDa subunit
• BTF2 p44
• BTF2
• BTF2P44TFIIH basal transcription factor complex p44 subunit
• General transcription factor IIH polypeptide 2
• general transcription factor IIH subunit 2
• general transcription factor IIH, polypeptide 2 (44kD subunit)
• general transcription factor IIH, polypeptide 2, 44kD subunit
• general transcription factor IIH, polypeptide 2, 44kDa
• MGC102806
• T-BTF2P44
• TFIIH

Background

Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: GTF2H2