GSK-3 alpha/beta Antibody (L5) - Azide and BSA Free

Western Blot: GSK3 alpha/beta Antibody [NBP2-29873] - HEK293 cell lysate was probed with anti-GSK3alpha /Beta (1:5,000 dilution). Arrows indicate GSK3alpha (~51kDa) and GSK3Beta (~46kDa).

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clone: L5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

GSK-3 alpha/beta Antibody (L5) - Azide and BSA Free Summary

• Description: Relevant Entrez Gene Numbers: NM_019884/NM_002093
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: N-terminal 6His-tagged recombinant full length human GSK3Beta.
• Specificity: GSK3 alpha and beta
  46kDa(beta)and51kDa(alpha) weight may be detected.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSK3A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Human, Mouse

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, and 30% glycerol.
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

Relevant Entrez Gene Numbers: NM_019884/NM_002093

Alternate Names for GSK-3 alpha/beta Antibody (L5) - Azide and BSA Free

• DKFZp686D0638
• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 alpha
• glycogen synthase kinase-3 alpha
• GSK-3 alpha
• GSK-3 alpha/beta

Background

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GSK3A
• Uniprot:
  • P49840()