GSAP Antibody

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse GSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8037) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for GSAP at approximately 98 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

GSAP was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Sheep Anti-Human/Mouse GSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8037) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

GSAP interacts with Fe65 to regulate APP phosphorylation&trafficking. (J) Co-IP analysis of GSAP (HA-tagged) with APP-C99 (Flag-tagged) in WT&Fe65KO (FKO) CAD cells. Representative data of two experiments. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34156424), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

GSAP Antibody Summary

• Immunogen: E. coli-derived recombinant mouse GSAP
  Arg737-Leu858
  Accession # Q3TCV3
• Specificity: Detects human and mouse GSAP in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PION
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSAP Antibody

• GSAP
• pigeon homolog (Drosophila)
• PION
• tcag7.1314

Background

GSAP (GSAP/gamma-Secretase Activating Protein; also known is Pion) is a member of the GSAP family of proteins. It is expressed in neurons, and purportedly serves as a regulator for g-secretase processing of APP. Initially, it was thought that cytosolic g-secretase mediated the second cleavage step in APP processing. In the presence of GSAP, APP (now C99 after b-secretase cleavage) was preferentially cleaved between either Val40-Ile41, or Ala42-Thr43, generating Ab40 and Ab42, respectively. In the absence of GSAP, APP would be preferentially cleaved between L49-Val50. Notably, the presence of GSAP was shown to have no effect on g-secretase processing of Notch. Subsequent studies have introduced uncertainty into these relationships. While an absence of GSAP does apparently reduce Ab production, its presence may not have the regulatory effect once proposed. Mouse GSAP-16K (15-17 kDa) is 121 amino acids (aa) in length (aa 738-858) (SwissProt #:Q3TCV3), it presumably represents a proteolytic cleavage product of the large 95-100 kDa, 858 aa GSAP-FL termed also Pion/pigeon homolog protein. There are no readily identifiable structural motifs associated with the molecule. Mouse PION has two isoform variants associated with the gene. One possesses a Phe substitution for aa 172-858, while another possesses a six aa substitution for aa 527-858. Over aa 737-858, mouse GSAP shares 94% and 88% aa sequence identity with rat and human GSAP, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GSAP Antibody (AF8037)(1)

Publication using AF8037

• Peng Xu, Jerry C. Chang, Xiaopu Zhou, Wei Wang, Michael Bamkole, Eitan Wong et al. GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer’s disease Journal of Experimental Medicine 2021-08-02 [PMID: 34156424]

Bioinformatics

• Gene Symbol: PION
• Uniprot:
  • Q3TCV3()