GRASP1 Antibody (OTI1E3) - Azide and BSA Free

Western Blot: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRASP1.

Immunocytochemistry/Immunofluorescence: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRASP1.

Immunohistochemistry: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Staining of paraffin-embedded Ovary tissue using anti-GRASP1 mouse monoclonal antibody.

Flow Cytometry: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Analysis of Hela cells, using anti-GRASP1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Immunocytochemistry/Immunofluorescence: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Staining of HepG2 cells using anti-GRASP1 mouse monoclonal antibody.

Immunohistochemistry: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Staining of paraffin-embedded breast tissue using anti-GRASP1 mouse monoclonal antibody.

Immunohistochemistry: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Staining of paraffin-embedded endometrium tissue using anti-GRASP1 mouse monoclonal antibody.

Immunohistochemistry: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Staining of paraffin-embedded Kidney tissue using anti-GRASP1 mouse monoclonal antibody.

Immunohistochemistry: GRASP1 Antibody (OTI1E3) - Azide and BSA Free [NBP2-72064] - Staining of paraffin-embedded prostate tissue using anti-GRASP1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

GRASP1 Antibody (OTI1E3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human GRIPAP1 (NP_064522) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIPAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GRASP1 Antibody (OTI1E3) - Azide and BSA Free

• DKFZp434P0630
• GRASP1
• GRASP-1MGC126595
• GRIP1 associated protein 1
• GRIP1-associated protein 1
• GRIPAP1
• KIAA1167MGC126593
• MPMGp800B12492Q3

Background

GRASP1 is a neuron-specific guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF) and is associated with the GRIP/AMPA receptor complex in brain (Ye et al., 2000 [PubMed 10896157]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIPAP1