Granzyme H Antibody (185813) [Alexa Fluor™ Plus 405]

• Reactivity: Hu
• Clone: 185813
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

Granzyme H Antibody (185813) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Granzyme D or rmGranzyme G is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme H Antibody (185813) [Alexa Fluor™ Plus 405]

• Cathepsin G-like 2
• CCP-X
• CCP-Xcytotoxic T-lymphocyte-associated serine esterase 1
• CGL2
• CGL-2
• CSP-Ccytotoxin serine protease-C
• CTLA1
• CTSGL2
• CTSGL2granzyme H
• Cytotoxic serine protease C
• Cytotoxic T-lymphocyte proteinase
• EC 3.4.21
• EC 3.4.21.-
• EC 3.4.21.79
• granzyme H (cathepsin G-like 2, protein h-CCPX)
• Granzyme H
• GrzH
• GZMH

Background

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1‑18), a propeptide (residues 19‑20) and a mature chain (residues 21‑246) (5‑7). After being activated by active cathepsin C, human Granzyme H cleaves a thioester substrate (8).

1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
3. Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581.
4. Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726.
5. Meier, M. et al. (1990) Biochemistry 29:4042.
6. Haddad, P. et al. (1991) Int. Immunol. 3:57.
7. Klein, J.L. et al. (1990) Tissue Antigens 35:220.
8. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.