GPR116 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPR116 Antibody [NBP3-14341] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPR116 Antibody [NBP3-14341] - Immunohistochemistry of formalin-fixed, paraffin-embedded human heart after heat-induced antigen retrieval.

• Reactivity: Hu, Pm
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

GPR116 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 20 amino acid peptide from internal region of human GPR116.
• Epitope: Internal
• Specificity: Human GPR116. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADGRF5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml..

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GPR116 Antibody - BSA Free

• FLJ90640
• G protein-coupled receptor 116
• GPR116
• Ig-Hepta homolog
• KIAA0758DKFZp564O1923
• KPG_001
• probable G-protein coupled receptor 116

Background

GPR116/KIAA0758 is an Orphan-B GPCR with an unknown ligand. GPR116 has two immunoglobulin-like repeats and a SEA box. RT-PCR analysis from the KIAA database suggests that KIAA0758 is expressed in heart, kidney, lung and ovary. ESTs have been isolated from brain, breast, eye, heart, kidney, liver, liver/spleen, lung, nasopharyngeal, spleen, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADGRF5