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VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
GPR116/KIAA0758 is an Orphan-B GPCR with an unknown ligand. GPR116 has two immunoglobulin-like repeats and a SEA box. RT-PCR analysis from the KIAA database suggests that KIAA0758 is expressed in heart, kidney, lung and ovary. ESTs have been isolated from brain, breast, eye, heart, kidney, liver, liver/spleen, lung, nasopharyngeal, spleen, and testis libraries.