gp130/CD130 Antibody (B11-B9-A8)

Western Blot: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Analysis of CD130 on Raji cell (1) and mouse lung tissue (2) lysates using anti-CD130 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Staining CD130 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-gp130/CD130 antibody. Counter stained with hematoxylin.

Western Blot: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Analysis of CD130 on CD130 recombinant protein using anti-CD130 antibody at 1/5,000 dilution.

Immunocytochemistry/Immunofluorescence: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Staining CD130 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Analysis of paraffin-embedded human liver tissue using anti-CD130 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Analysis of paraffin-embedded human kidney tissue using anti-CD130 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: gp130/CD130 Antibody (B11-B9-A8) [NBP2-89120] - Analysis of paraffin-embedded human pancreas tissue using anti-CD130 antibody. Counter stained with hematoxylin.

Product Discontinued

gp130/CD130 Antibody (B11-B9-A8) Summary

• Immunogen: Recombinant protein within Human gp130/CD130 238-422 / 918. (SwissProt: P40189 Human)
• Localization: Cell membrane.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6ST
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for gp130/CD130 Antibody (B11-B9-A8)

• CD130 antigen
• CD130
• CDw130
• DKFZp564F053
• gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
• gp130
• IL-6 receptor subunit beta
• IL-6R subunit beta
• IL-6RB
• IL-6R-beta
• IL6ST
• interleukin 6 signal transducer (gp130, oncostatin M receptor)
• interleukin receptor beta chain
• interleukin-6 receptor subunit beta
• Interleukin-6 signal transducer
• Membrane glycoprotein 130
• membrane glycoprotein gp130
• Oncostatin-M receptor subunit alpha

Background

CD130 is encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL6ST