Golgin 97 Antibody (6G3) - Azide and BSA Free

Western Blot: Golgin 97 Antibody (6G3) [H00002800-M01] - GOLGA1 monoclonal antibody (M01), clone 6G3 Analysis of GOLGA1 expression in HeLa.

Sandwich ELISA: Golgin 97 Antibody (6G3) [H00002800-M01] - Detection limit for recombinant GST tagged GOLGA1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 6G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Golgin 97 Antibody (6G3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GOLGA1 (NP_002068, 668 a.a. ~ 767 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKPGPEMANMAPSVTNNTDLTDAREINFEYLKHVVLKFMSCRESEAFHLIKAVSVLLNFSQEEENMLKETLEYKMSWFGSKPAPKGSIRPSISNPRIPWS
• Localization: Golgi Apparatus
• Marker: TGN Marker
• Specificity: GOLGA1 - golgi autoantigen, golgin subfamily a, 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GOLGA1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Golgin 97 Antibody (6G3) - Azide and BSA Free

• gap junction protein, alpha 4, 37kD
• golgi autoantigen, golgin subfamily a, 1
• golgin A1
• Golgin subfamily A member 1
• Golgin-97
• MGC33154

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Golgin 97 Antibody (H00002800-M01)(1)

Publication using H00002800-M01

• Teoh KT, Siu YL, Chan WL et al. The SARS Coronavirus E Protein Interacts with PALS1 and Alters Tight Junction Formation and Epithelial Morphogenesis. Mol Biol Cell. 2010-09-22 [PMID: 20861307]

Bioinformatics

• Gene Symbol: GOLGA1
• Entrez:
  • 2800(Human)
• OMIM:
  • 602502(Human)
• Uniprot:
  • NP_002068(Human)