GM-CSFR alpha Antibody (1064818) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 1064818
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

GM-CSFR alpha Antibody (1064818) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human GM-CSF Ralpha
  Met1-Gly320
  Accession # P15509
• Specificity: Detects human GM-CSF in direct ELISA.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GM-CSFR alpha Antibody (1064818) [Alexa Fluor® 750]

• CD116 antigen
• CD116
• CDw116
• colony stimulating factor 2 receptor alpha subunit
• colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage)
• CSF2R
• CSF2RA
• CSF2RAX
• CSF2RAY
• CSF2RX
• CSF2RY
• GM-CSF R alpha
• GM-CSF receptor alpha subunit
• GMCSFR alpha
• GMCSFR
• GM-CSFRa
• GM-CSF-R-alpha
• GMCSFR-alpha
• GMR
• GMR-alpha
• granulocyte-macrophage colony-stimulating factor receptor alpha chain
• granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• MGC3848
• MGC4838

Background

Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1‑4). Mature human GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSxWS motif, a 26 aa transmembrane segment, and a 54 aa cytoplasmic domain (5). Within the ECD, human GM-CSF R alpha shares approximately 33% aa sequence identity with mouse and rat GM-CSF R alpha . Alternative splicing of human GM-CSF R alpha generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non-hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta _c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta _c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9‑11). The shared usage of beta _c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.