Glypican 1 Antibody

Western Blot: Glypican 1 Antibody [H00002817-A01] - Detection against Immunogen (37.99 KDa) .

Product Discontinued

Glypican 1 Antibody Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: GPC1 (NP_002072, 24 a.a. - 131 a.a.) partial recombinant protein with GST tag. DPASKSRSCGEVRQIYGAKGFSLSDVPQAEISGEHLRICPQGYTCCTSEMEENLANRSHAELETALRDSSRVLQAMLATQLRSFDDHFQHLLNDSERTLQATFPGAFG
• Specificity: GPC1 - glypican 1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: GPC1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: The quality control of this antibody is limited to WB on the immunizing protein. It has been used for ELISA. Abnova's recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 50 % glycerol
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glypican 1 Antibody

• FLJ38078
• Glypican 1
• glypican proteoglycan 1
• glypican
• glypican-1
• GPC1

Background

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glypican 1 Antibody (H00002817-A01)(2)

Publications using H00002817-A01

• Wu HY, Chang YH, Chang YC, Liao PC et al. Proteomics analysis of nasopharyngeal carcinoma cell secretome using a hollow fiber culture system and mass spectrometry. J Proteome Res. 2009-01-01 [PMID: 19012429]
• Payne CK, Jones SA, Chen C et al. Internalization Trafficking of Cell Surface Proteoglycans Proteoglycan-Binding Ligs. Traffic;8(4):389-401. 2007-04-01 [PMID: 17394486]

FAQs for Glypican 1 Antibody (H00002817-A01). (Showing 1 - 1 of 1 FAQs).

1. Is this antibody react with H00002817-Q01? I want to use it for ELISA.
   • The immunogen for this product falls between aa 130-170 of the human glypican protein. The protein H00002817-Q01 is made to a sequence from aa 24-132. Therefore, I would not recommend the use of this antibody with this protein. We do have one antibody validated in ELISA that was made to the same immunogen range as the protein H00002817-Q01.

Bioinformatics

• Gene Symbol: GPC1
• Entrez:
  • 2817(Human)
• OMIM:
  • 600395(Human)
• Uniprot:
  • NP_002072()