Glycoprotein V/CD42d Antibody (1C2) - BSA Free

Flow Cytometry: GPV Antibody (1C2) [NBP2-00071] - Staining of mouse platelets with Anti-Mouse CD41 FITC and 0.125 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.125 ug of Anti-Mouse/Rat CD42d Purified (right) followed by Anti-Armenian Hamster IgG PE . Total events were used for analysi

• Reactivity: Mu, Rt
• Applications: Flow, IHC, IP
• Clone: 1C2
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Format: BSA Free

Glycoprotein V/CD42d Antibody (1C2) - BSA Free Summary

• Description: Novus Biologicals Armenian Hamster Glycoprotein V/CD42d Antibody (1C2) - BSA Free (NBP2-00071) is a monoclonal antibody validated for use in IHC, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody is CD42d (platelet glycoprotein V).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: GP5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: This 1C2 antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 1C2 antibody has also been reported for use in immunoprecipitation, and immunohistology staining of frozen tissue sections. t is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Glycoprotein V/CD42d Antibody (1C2) - BSA Free

• platelet glycoprotein V
• CD42d
• glycoprotein V (platelet)
• Glycoprotein V
• GP5
• GPV

Background

The monoclonal antibody 1C2 recognizes mouse and rat CD42d, a single transmembrane protein also known as platelet glycoprotein V (GPV). CD42d interacts with CD42a, CD42b and CD42c to form the receptor for von willebrand factor and thrombin. This functional complex plays a role in platelet adhesion. Expression of CD42d (unlike other markers such as CD41) is restricted to resting platelets and megakaryocytes and therefore is a good marker for development of megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GP5