Glycine Receptor Alpha 1 Antibody

Western Blot: Glycine Receptor Alpha 1 Antibody [NBP2-26171] - Staining of Mouse Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Glycine Receptor Alpha 1 Antibody Summary

• Immunogen: Peptide with sequence CLQAKDGISVKGAN corresponding to internal region according to NP_037265.1.
• Specificity: The immunizing peptide represents part of the cytoplasmic domain. The antibody is not expected to cross-react with other glycine receptor alpha proteins.
• Predicted Species: Human (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GLRA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in mouse and rat testis lysates (calculated MW of 51.7 kDa band according to mouse NP_065238.2). The observed molecular weight corresponds to the glycosylated form.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glycine Receptor Alpha 1 Antibody

• GLRA 1
• GLRA1
• Glycine receptor 48 kDa subunit
• Glycine receptor strychnine-binding subunit
• glycine receptor subunit alpha-1
• glycine receptor, alpha 1 (startle disease/hyperekplexia)
• glycine receptor, alpha 1
• HKPX1
• MGC138878
• MGC138879
• STHE

Background

Glycine is an important inhibitory neurotransmitter in the mammalian central nervous system, especially in the brainstem and spinal cord. It acts by binding to anion-conducting glycine receptors that belong to a superfamily of ligand-gated ion channels. Glycine receptors were first purified as strychnine binding sites in membrane fractions of adult spinal cord from rats (1). These strychnine-sensitive binding sites are different from the strychnine-insensitive binding sites found in the N-methyl-D-aspartate (NMDA) subtype of glutamate receptors. Adult glycine receptors are heterodimers composed of two subunits of 48 kDa and 58 kDa, denoted as a1 and b subunits. Fetal glycine receptors are homomers composed of a2 subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glycine Receptor Alpha 1 Antibody (NBP2-26171)(1)

Publication using NBP2-26171

• Wang Q, Lynch JW. Activation and desensitization induce distinct conformational changes at the extracellular-transmembrane domain interface of the glycine receptor. J Biol Chem 2011-11-04 [PMID: 21917927]

Bioinformatics

• Gene Symbol: GLRA1
• Entrez:
  • 2741(Human)
  • 14654(Mouse)
  • 25674(Rat)