Glutathione Peroxidase 4/GPX4 Antibody (EPNCIR144)

Product Discontinued

Glutathione Peroxidase 4/GPX4 Antibody (EPNCIR144) Summary

• Immunogen: A synthetic peptide corresponding to residues in mouse GPX4 was used as an immunogen.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPX4
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 100
  • Immunohistochemistry 1:100 - 1:250
  • Immunohistochemistry-Paraffin 1:100 - 250
  • Immunoprecipitation 1:10 - 100
  • Western Blot 1:10000-50000
• Application Notes: This product is useful in Western Blot,Immunohistochemistry,Immunocytochemistry,Immunohistochemistry,Immunoprecipitation,
  Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:50. Apparent MW in kDa on Simple Western was kDa.

Reactivity Notes

Mouse cross reactivity tested by western blot and Immunohistochemistry, Immunohistochemistry-Paraffin tested for use on mouse tissues. Rat cross reactivity tested by Western Blot only.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Glutathione Peroxidase 4/GPX4 Antibody (EPNCIR144)

• EC 1.11.1
• EC 1.11.1.12
• glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Glutathione Peroxidase 4
• GPX4
• GPx-4
• GSHPx-4
• MCSP
• PHGPx
• PHGPxsnGPx
• phospholipid hydroperoxidase
• phospholipid hydroperoxide glutathione peroxidase, mitochondrial
• snGPx
• snPHGPx
• sperm nucleus glutathione peroxidase

Background

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. GPX4 localizes to the nucleus, mitochondrion, and cytoplasm (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPX4
• Entrez:
  • 2879(Human)
• Uniprot:
  • O70325(Human)