Glutathione Peroxidase 1/GPX1 Assay Kit (Colorimetric)

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Glutathione Peroxidase 1/GPX1 Assay Kit (Colorimetric) [KA0882] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time ...read more

Product Details

Summary
Reactivity Rt, Ma, RbSpecies Glossary
Applications Func, Quantification
Suitable Sample Type
Cell Lysates, Erythrocyte Lysates, Plasma, Tissue Homogenates
Sensitivity
0.5 mU/mL

Order Details

Glutathione Peroxidase 1/GPX1 Assay Kit (Colorimetric) Summary

Description
Glutathione Peroxidase Assay Kit is used for measuring glutathione peroxidase (GPx) activity.
Sensitivity
0.5 mU/mL
Assay Type
Colorimetric
Sample Volume
2-50 uL
Kit Type
Assay Kit (Colorimetric)
Gene
GPX1

Applications/Dilutions

Publications
Read Publications using KA0882.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. GPx Assay Buffer
  2. NADPH (lyophilized)
  3. Glutathione Reductase
  4. Glutathione (GSH; lyophilized)
  5. Cumene Hydroperoxide
  6. GPx Positive Control (lyophilized)

Notes

Quality control test: Standard curve

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Glutathione Peroxidase 1/GPX1 Assay Kit (Colorimetric)

  • Cellular glutathione peroxidase
  • CGPx
  • EC 1.11.1
  • EC 1.11.1.9
  • Glutathione Peroxidase 1
  • GPX1
  • GPx-1
  • GSHPX1
  • GSHPx-1
  • MGC14399
  • MGC88245

Background

The Glutathione Peroxidase family of enzymes play important roles in the protection of organisms from oxidative damage. GPx converts reduced glutathione (GSH) to oxidized glutathione (GSSG), to reduce lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. In this Glutathione Peroxidase Assay Kit, GPx reduces Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH (easily measured at 340 nm) is proportional to GPx activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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KA0882
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Publications for Glutathione Peroxidase 1/GPX1 Kit (KA0882)(2)

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Bioinformatics

Gene Symbol GPX1