CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Glutathione Peroxidase 1/GPX1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3798) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3798-SP | |||
HAF017). A specific band was detected for Glutathione Peroxidase 1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
HAF017). A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 24 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # 
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Western-Blot-NB100-40805-img0004.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Frozen: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of PFA-perfused cryosection of Porcine Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Frozen-NB100-40805-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Paraffin-NB100-40805-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Immunohistochemistry-Paraffin-NB100-40805-img0002.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of various lysates, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-25943_rabbit-glutathione-peroxidase-1-gpx1-mab-arc53251-62202415421913.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of various lysates, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Analysis of Rat liver, using Glutathione Peroxidase 1/GPX1 antibody at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-25943_rabbit-glutathione-peroxidase-1-gpx1-mab-arc53251-6220241542197.jpg)
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (ARC53251) [NBP3-25943] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glutathione Peroxidase 1/GPX1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-25943_rabbit-glutathione-peroxidase-1-gpx1-mab-arc53251-52202511411444.jpg)
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody [Glutathione Peroxidase 1/GPX1] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Glutathione Peroxidase 1/GPX1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-25943_rabbit-glutathione-peroxidase-1-gpx1-mab-arc53251-52202517333822.jpg)
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-2982024149266.jpg "Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution.<br/><br/> Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-292024119233.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32389, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-29202416372194.jpg)
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32388) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-298202414161846.jpg "Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32388) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution.<br/><br/> Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32388) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-29202410234266.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (A6-C0-B9-R) [NBP3-32388] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32388, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32388_mouse-glutathione-peroxidase-1-gpx1-mab-a6-c0-b9-r-2920241645329.jpg)
![ELISA: Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68014] - Samples were spiked with high concentrations of Human Glutathione Peroxidase 1/GPX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68014_human-glutathione-peroxidase-1-gpx1-elisa-kit-colorimetric-132202416235755.jpg "ELISA: Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68014] - Samples were spiked with high concentrations of Human Glutathione Peroxidase 1/GPX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68014] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68014_human-glutathione-peroxidase-1-gpx1-elisa-kit-colorimetric-432024903511.jpg)
![ELISA: Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68015] - Samples were spiked with high concentrations of Mouse Glutathione Peroxidase 1/GPX1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-68015_mouse-glutathione-peroxidase-1-gpx1-elisa-kit-colorimetric-13220241601217.jpg)
![ELISA: Mouse Glutathione Peroxidase 1/GPX1 ELISA Kit (Colorimetric) [NBP2-68015] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-68015_mouse-glutathione-peroxidase-1-gpx1-elisa-kit-colorimetric-432024932654.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26156] - Staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Western-Blot-NBP2-26156-img0001.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26156] - Staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (9E5H3) [NBP3-33354] - Western blot analysis of lysates from Rat liver, using Glutathione Peroxidase 1/GPX1 Rabbit mAb at 1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33354_rabbit-glutathione-peroxidase-1-gpx1-mab-9e5h3-912202414201385.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (9E5H3) [NBP3-33354] - Western blot analysis of lysates from Rat liver, using Glutathione Peroxidase 1/GPX1 Rabbit mAb at 1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.")
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (9E5H3) [NBP3-33354] - Western blot analysis of various lysates, using Glutathione Peroxidase 1/GPX1 Rabbit mAb at 1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-33354_rabbit-glutathione-peroxidase-1-gpx1-mab-9e5h3-912202415175212.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26155] - Staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutathione-Peroxidase-1-GPX1-Antibody-Western-Blot-NBP2-26155-img0001.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26155] - Staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![SDS-Page: Recombinant Human Glutathione Peroxidase 1/GPX1 Protein [NBP1-72512] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 24.2kDa (223aa), confirmed by MALDI-TOF](http://images.novusbio.com/fullsize/Recombinant-Human-Glutathione-Peroxidase-1-GPX1-Protein-SDS-Page-NBP1-72512-img0001.jpg "SDS-Page: Recombinant Human Glutathione Peroxidase 1/GPX1 Protein [NBP1-72512] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. Molecular Weight: 24.2kDa (223aa), confirmed by MALDI-TOF")
