Glutathione Peroxidase 1/GPX1 Antibody

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NBP2-26156] - Staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Glutathione Peroxidase 1/GPX1 Antibody Summary

• Immunogen: Peptide with sequence C-DYTEMNDLQKRLGPR corresponding to internal region according to NP_032186.2.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in rat kidney and liver lysates and in mouse kidney lysates (calculated MW of 22.3 kDa band according to NP_032186.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

FUNCTION: Protects the hemoglobin in erythrocytes from oxidative breakdown. CATALYTIC ACTIVITY: 2 glutathione + H2O2 = glutathione disulfide + 2 H2O. SUBUNIT: Homotetramer. SUBCELLULAR LOCATION: Cytoplasm. SIMILARITY: Belongs to the glutathione peroxidase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 1/GPX1 Antibody (NBP2-26156)(1)

Publication using NBP2-26156

• Lubos E, Kelly NJ, Oldebeken SR et al. Glutathione peroxidase-1 deficiency augments proinflammatory cytokine induced redox signaling and human endothelial cell activation. J Biol Chem 2011-10-14 [PMID: 21852236]

Bioinformatics

• Gene Symbol: GPX1
• Entrez:
  • 14775(Mouse)
  • 24404(Rat)