Glutathione Peroxidase 1/GPX1 Antibody

Western Blot: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Frozen: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of PFA-perfused cryosection of Porcine Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Glutathione Peroxidase 1/GPX1 Antibody [NB100-40805] - Staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Po
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Glutathione Peroxidase 1/GPX1 Antibody Summary

• Description: Novus Biologicals Goat Glutathione Peroxidase 1/GPX1 Antibody (NB100-40805) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Glutathione Peroxidase 1/GPX1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-REALPAPSDDATA corresponding to internal region according to NP_000572.2.
• Specificity: This antibody is expected to recognise isoform 1 ( NP_000572.2) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GPX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.7 ug/ml
  • Immunohistochemistry-Frozen 20 ug/ml
  • Immunohistochemistry-Paraffin 3.7 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in human liver and kidney lysates (calculated MW of 22.1 kDa band according to NP_000572.2). IHC: Human cerebral cortex shows staining of microglia. Paraffin embedded Human Liver. IHC-Fr: Porcine kidney shows staining of cytoplasm in PCT epithelial cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody

• Cellular glutathione peroxidase
• CGPx
• EC 1.11.1
• EC 1.11.1.9
• Glutathione Peroxidase 1
• GPX1
• GPx-1
• GSHPX1
• GSHPx-1
• MGC14399
• MGC88245

Background

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 1/GPX1 Antibody (NB100-40805)(1)

Publication using NB100-40805

• Kato S, Kato M, Abe Y et al. Redox system expression in the motor neurons in amyotrophic lateral sclerosis (ALS): immunohistochemical studies on sporadic ALS, superoxide dismutase 1 (SOD1)-mutated familial ALS, and SOD1-mutated ALS animal models. Acta Neuropathol 2005-06-28 [PMID: 15983830]

Bioinformatics

• Gene Symbol: GPX1
• Entrez:
  • 2876(Human)
• Uniprot:
  • NP_000572.2(Human)