Glut5 Antibody (195205) [Unconjugated]

THP-1 cells were stained with Mouse Anti-Human Glut5 Monoclonal Antibody (Catalog # MAB1349, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

NS0 mouse myeloma cell line either transfected with human Glut5 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human Glut5 Monoclonal Antibody, followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 195205
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Glut5 Antibody (195205) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Glut5
  Met1-Gln501
  Accession # P22732
• Specificity: Detects human Glut5. Stains human Glut5 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC2A5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glut5 Antibody (195205) [Unconjugated]

• Fructose transporter
• Glucose transporter type 5, small intestine
• Glut5
• GLUT-5
• GLUT5glucose transporter-like protein 5
• SLC2A5
• solute carrier family 2 (facilitated glucose transporter), member 5
• solute carrier family 2 (facilitated glucose/fructose transporter), member 5
• solute carrier family 2, facilitated glucose transporter member 5

Background

Glut5 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues. Glut5 is expressed at highest levels in small intestine and at lower levels in kidney, testis, skeletal muscle, and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis, and other tissues.

1. Kayano, T. et al. (1990) J. Biol. Chem. 265:13276.
2. Corpe, C.P. (2002) Biochem. Biophys. Acta. 1576:191.
3. Sasaki, A. et al. (2003) Neurosci. Lett. 338:17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glut5 Antibody (MAB1349)(3)

Publications using MAB1349

• Emily Maguire, Georgina E Menzies, Thomas Phillips, Michael Sasner, Harriet M Williams, Magdalena A Czubala et al. PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease‐protective variant PLC gamma 2 R522 The EMBO Journal 2021-09-01 [PMID: 34254352] (Immunocytochemistry, Human)
• Hugo Pontes Antunes, Ricardo Teixo, João André Carvalho, Miguel Eliseu, Inês Marques, Ana Mamede et al. Diabetes mellitus and prostate cancer metabolism: Is there a relationship? Archivio Italiano di Urologia e Andrologia 2018-09-30 [PMID: 30362687]
• Ana F. Brito, Ana M. Abrantes, Marina Ribeiro, Rui Oliveira, João Casalta-Lopes, Ana C. Gonçalves et al. Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression Journal of Clinical and Experimental Hepatology 2015-09-01 [PMID: 26628835]

Bioinformatics

• Gene Symbol: SLC2A5
• Uniprot:
  • P22732()